Characterization and Comparison of Mycobacterial Antigens by Two-Dimensional Immunoelectrophoresis
نویسندگان
چکیده
منابع مشابه
Characterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis.
Two-dimensional immunoelectrophoresis (2D-IEP), in which a complex of antigens is subjected to electrophoresis first through an agarose matrix in one direction and secondly through an antiserum-agarose matrix at right angles to the first direction, was evaluated as a tool for analysis of mycobacterial antigens. Cell extracts from four species of mycobacteria, Mycobacterium tuberculosis (four st...
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Two-dimensional immunoelectrophoresis was employed to electrophoretically identify membrane and cytoplasmic antigens of Mycoplasma arginini G-230. Five distinct cytoplasmic antigens were observed in soluble fractions prepared by digitonin lysis with electrophoretic mobilities (relative to bovine albumin) ranging from 0.36 to 0.86; four of these were common to other M. arginini strains: leonis a...
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چکیده ندارد.
15 صفحه اولCharacterization and comparison of mycobacterial antigens by discontinuous pore gradient gel electrophoresis.
Concentrated culture filtrates and cell extracts were prepared from selected mycobacteria and analyzed by a multistage polyacrylamide electrophoretic procedure. Various staining procedures were used to detect protein, carbohydrate, nucleic acid, and lipid constituents. Visual examination of the more prominently stained components indicated differences and similarities in each species of Mycobac...
متن کاملSerological comparison of five arginine-utilizing Mycoplasma species by two-dimensional immunoelectrophoresis.
Five arginine-utilizing, nonglycolytic Mycoplasma species were compared serologically by two dimensional immunoelectrophoresis. The survey included: Mycoplasma hominis, Mycoplasma arthritidis, Mycoplasma gateae, Mycoplasma gallinarum, and three strains of Mycoplasma arginini. Although the M. arginini strains showed strong cross-reactions, each strain produced a different antigenic profile which...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1972
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.6.4.564-573.1972